Fast solid support detection of PCR amplified viral DNA sequences using radioiodinated or hapten labelled primers
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چکیده
منابع مشابه
Amplicon: software for designing PCR primers on aligned DNA sequences
SUMMARY Amplicon is a program for designing PCR primers on aligned groups of DNA sequences. The most important application for Amplicon is the design of 'group-specific' PCR primer sets that amplify a DNA region from a given taxonomic group but do not amplify orthologous regions from other taxonomic groups. AVAILABILITY Amplicon is freely available as a script that will run on any platform wi...
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BACKGROUND Assessment of the biodiversity of communities of small organisms is most readily done using PCR-based analysis of environmental samples consisting of mixtures of individuals. Known as metagenetics, this approach has transformed understanding of microbial communities and is beginning to be applied to metazoans as well. Unlike microbial studies, where analysis of the 16S ribosomal DNA ...
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We observed that single-stranded DNA adsorbs on negatively charged gold nanoparticles (Au-nps) and the adsorption rate is sequence length dependent. We also found that the adsorption stabilizes the Au-nps against salt-induced aggregation. Based on these observations, we developed a simple, rapid and cost efficient colorimetric assay to identify specific sequences in PCR amplified DNA. The assay...
متن کاملSequencing of PCR-amplified DNA.
Alternatives for sequencing of PCR products essentially fall into one of two categories; generation of single-stranded DNA for sequencing or the direct sequencing of double-stranded product. Of the two alternatives, sequencing of double-stranded PCR products is likely to be of greatest immediate significance in terms of general applicability and rapidity. Double-stranded sequencing allows the u...
متن کاملEnhanced solid phase PCR: mechanisms to increase priming by solid support primers.
Conventional solid phase amplification regimens such as solid phase PCR (SP-PCR), asymmetric SP-PCR, and bridge PCR are mechanistically limited with respect to amplification efficiency and solid support primer involvement. Here we present enhanced solid phase PCR (ESP-PCR) in which solid support primer priming is facilitated by its nesting and high melting temperature (T(m)) relative to the aqu...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1990
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/18.11.3175